通用引物

引物名称	序列
pmirGLO-T5-seqF	GGACGAGGTGCCTAAAGGAC
pGL3-T5-seqR(-100)	gCCTTATgCAgTTgCTCTCC
pGL3-T5-seqF(-100)	ACTAACATACGCTCTCCATC
Pet28a-T5-seqR	ACCCCTCAAGACCCGTTTAG
PCDH130-seq-R	CACCCGTTCAATTGCCGACC
pLVmini-seq-R	AGCAGATCTTATCTTCGTTG
YM017	CATTTTCAgggATAgCAAgC
YM016	TCACACAggAAACAgCTATg
YFP-NC	AgATCTggCggAggAggTTC
YFP-cc	ggATCCCATTgTACAgCTCgTCCATgCC
WPRE-R	ggCATTAAAgCAgCgTATCC
ubic-r	TTCgTCTgACgTggCAgCgCT
ubic-f	TgTACCTATCTTCTTAAgTAgCTgAAgCTCC
Ubi	TTgTCgATgCTCACCCTgTTg
U6	ATggACTATCATATgCTTACCgTA
T7ter	gCTAgTTATTgCTCAgCgg
T7 eev promotor	AAggCTAgAgTACTTAATACgA
T7	TAATACgACTCACTATAgg
T3	ATTAACCCTCACTAAAgggA
SV40-promoter	gCCCCTAACTCCgCCCATCC
SV40-PArev	CCTCTACAAATgTggTATgg
SV40-CX-L(chimeng)	TTTTATgTTTCAggTTCAgg
Sq-FP-chimeng	gAAggTTgTggATCTggATA
SP-Rzengdongchang	TgCAATAACTTCgTATAggC
Sp-L1zengdongchang	gCggTgTCATCTATgTTACTAg
SP6	ATTTAggTgACACTATAg
shpDown-F	AgCTACATTTTACATgATAggCTT
SFB-RP	ATTTAgCAgCAgCggTTTCT
SFB-FP	gTCCACTCCCAggTCCAACT
SeqF-chimeng	ATTTTTgTgATgCTCgTCAg
S.tag	CgAACgCCAgCACATggACA
RVP4	gACgATAgTCATgCCCCgCg
RVP3R	CgTCTTCgAgTgggTAgAATg
RVP3	CTAgCAAAATAggCTgTCCC
RV-M	gAgCggATAACAATTTCACACAgg
RREUP(chen)	CTgCTgCTgCACTATACCAgA
RREdown(chen)	TCTggTATAgTgCAgCAgCAg
RFP-5-primer	TACTCAgATCTCgATATCgAA
RFP-3-primer	gATCAgTTATCTAgATCCggT
pYES2-F	TAATATACCTCTATACTTTAACgTC
pYES2.R	TCggTTAgAgCggATgTg
PX330-SEQ-F	TATTTCCCATgATTCCTTCATATTTgC
pUp-pDown-flank-r	AATACgACTCACTATAggggA
pUp-pDown-flank-f	CggCCAgTCTTAAgCTCggg
PUC57-seq-R	CTggCACgACAggTTTCCC
pUC57-seq-F	ACTgTTgggAAgggCgATC
PUC57-PGK-SEQ-R	ACCggTggATgTggAATgTgTg
PUC57-PGK-SEQ-F	CATTCTgCACgCTTCAAAAgCg
pUC510R	TTCCggCTCgTATgTTgTgT
pUC344	gTgCTgCAAggCgATTAAgT
PUAST	gCTCACCTgTgATTgCTCCT
Psin-Ef1a(panzu)	AgTTgCgTgAgCggAAAgATgg
Psicheck2.R	gCCgCCTCCgAATgAgAgTg
Psicheck2.F	TgAAgggCCTCCACTTCAgC
pSG5-3	gAAATTTgTgATgCTATTgC
PQE30R	gTTCTgAggTCATTACTgg
PQE30F	TgAgCggATAACAATTTCAC
PMX-PR	TTATCgTCgACCACTgTgCTg
PMX-PF	gACggCATCgCAgCTTggAT
PMSCV-5	CCCTTgAACCTCCTCgTTCgACC
PMSCV-3	gAgACgTgCTACTTCCATTTgTC
pMAL-C2X-R	CTgCAAggCgATTAAgTTgg
pMAL-C2X-F	TgCgTACTgCggTgATCAAC
pLXSN-R	gTTgCTgACTAATTgAgATg
pLXSN-F	CTTgAACCTCCTCgTTCgAC
plv-final-internal-r	AgCCTgCTTTTTTgTACAAACTTg
plv-final-internal-f	CAAgTTTgTACAAAAAAgCAggCT
pLV(KD)-Puro-seq-PF3	gTggATCTCTgCTgTCCCTgTAAT
pLNCX-R	ACCTACAggTggggTCTTTCATTCCC
pLNCX-F	AgCTCgTTTAgTgAACCgTCAgATC
pLNCX2-R	ACCTACAggTggggTCTTTCATTC
pLKO-EGFP-seqR	gTTTACCgTAAATACTCCAC
plko.1.seqR	gCggCCAAAgTggATCTCTg
pLKO.1 puro-seq-PF(62.1)	gAgggCCTATTTCCCATgATTC
plenti-seq-R	CTTTACATggTCggAAATgC
plenti-seq-F	CCgCTTCCTTCTACATgTTACC
pl-cn-R	CACgACATTCAACAgACCT
pl-cc-R	CACATTgCCAAAAgACgg
pJET1.2 reverse	AAgAACATCgATTTTCCATggCAg
pJET1.2 forward	CgACTCACTATAgggAgAgCggC
PITES2-EGFP-5	gTAggCgTgTACggTgggAg
PIRES3-EGFP-R	AACgCACACCggCCTTATTC
pGL3-T5-seqR	CTTATgCAgTTgCTCTCCAg
pGL3-T5-seqF	gCgggCCTCTTCgCTATTAC
PGK-FP	CCTTCgCTTTCTgggCTCA
PGK-charu-R(panguangjinzu)	ACCggTggATgTggAATgTgTg
PGK3	ACTTgTgTAgCgCCAAgTg
PGEX-F	TATTCTgAAATgAgCTgTTgAC
PGEX-5	gggCTggCAAgCCACgTTTggTg
PGEX-3	CCgggAgCTgCATgTgTCAgAgg
pGAP-F(nie)	gTCCCTATTTCAATCAATTgAA
pFastBacR	CAAATgTggTATggCTgATT
pFastBacF	ggATTATTCATACCgTCCCA
pET-R	gCTAgTTATTgCTCAgCgg
pET-F2	TggCCAggACCCAACgCTgC
pET-F	ATgCgTCCggCgTAgAggAT
pEGFP-N-5	TgggAggTCTATATAAgCAgAg
PEGFP-N-3(EGFP-Nrev)	CgTCgCCgTCCAgCTCgACCAg
pEGFP-C-5(EGFP-cfor)	CATggTCCTgCTggAgTTCgTg
pEGFP-C-3	TATggCTgATTATgATCAgT
pEAQ-R	ACTCTgAgCACAgAAAACCg
pEAQ-F	TgTTACgATTCTgCTgACTTTC
PDONR-R	gTAACATCAgAgATTTTgAgACAC
PDONR-F	TCgCgTTAACgCTAgCATggATCTC
PDC315-R	CgATgCTAgACgATCCAg
PDC315-F	ACgTgggTATAAgAggCg
Pcold-R	TggCAgggATCTTAgATTCTg
Pcold-F	ACgCCATATCgCCgAAAgg
PCMV-R	TCCAAACTCATCAATgTATC
PCMV-F	TCTAAAAgCTgCggAATTgT
PCMV6.F	TTgTTggTCATgCggCCCTg
pcDNA3.1-T5-R	CCAgggTCAAggAAggCACg
PCDNA3.1R(BGH)	TAgAAggCACAgTCgAgg
PCDNA3.1F	CTAgAgAACCCACTgCTTAC
pCDH-EF1a-R(xiechen)	ACACgACATCACTTTCCCAg
pCAGGS-seqR	AAAATTCCAACACACTATTgCAATg
pCAGGS-seqF	AgAgCCTCTgCTAACCATgTTCATg
pB-XhoI-seq-FP	TgAACACgTCgCTAAgCgAA
pB-XbaI-seq-RP	gCCTCATCATCACTAgATgg
PBV220R	CTgCgTTCTgATTTAATCTg
PBV220F	AAgAAgggCAgCATTCAAAg
pBD-GAL4-R	AAgAgTTACTCAAgAACAAgAA
pBD-GAL4-F	gCCTCTAACATTgAgACAgC
pBAD-R1	AACAgCCAAgCTggAgACC
pBAD-R	gATTTAATCTgTATCAgg
pBAD-F1	TTTATCCATAAgATTAgCgg
pBAD-F	ATgCCATAgCATTTTTATCC
pBaBe-5	CTTTATCCAgCCCTCAC
pBaBe-3	ACCCTAACTgACACACATTCC
OpIE2Reverse	gACAATACAAACTAAgATTTAgTCAg
OpIE2Forward	CgCAACgATCTggTAAACAC
NS8	TCCgCAggTTCACCTACggA
NS1	gTAgTCATATgCTTgTCTC
NOS	ACCggCAACAggATTCAATC
NA-F(dongyangguang)	gCgCCACCAgACATAATAgC
MT-forward	CATCTCAgTgCAACTAAA
Mouse-u6	CAgCACAAAAggAAACTCACC
MO2.F	CAgCCTCCggACTCTAgC
mfe-seq	TTggAggTCgCTgAgTAgTg
mcherry-R	AgggAggAgTCCTgggTCAC
malEprimer	ggTCgTCAgACTgTCgATgAAgCC
M13R(-48)	AgCggATAACAATTTCACACAggA
M13R	CAggAAACAgCTATgACC
M13F(-47)	CgCCAgggTTTTCCCAgTCACgAC
M13F	TgTAAAACgACggCCAgT
Lig3P3(chen)	CAggCTAAgTTgACAACCACT
LentiD10A-F-chimeng	CAgTACAATCTgCTCTgATg
ITS5	ggAAgTAAAAgTCgTAACAAgg
ITS4	TCCTCCgCTTATTgATATgC
ITS1	TCCgTAggTgAACCTgCgg
IRESrev	CCTCACATTgCCAAAAgACg
hybirdintronF(chen)	TgTAATTAgCTgAgCAAgAg
HIV-1up(chen)	CgAgTCCTgCgTCgAgAgAg
hGH-pA-R	CCAgCTTggTTCCCAATAgA
H1primer	CgCTATgTgTTCTgggAAAT
GLP2	CTTTATgTTTTTggCgTCTTCCA
GLP1	TgTATCTTATggTACTgTAACTg
GAL4ADfor	TACCACTACAATggATg
FUW-seq-F	CgggTTTATTACAgggACAgC
F86-CX-L-chimeng	ACCTggCCATCTCTgACCTg
espcas-down-F(chen)	CACggATCgACCTgTCTCAgC
EFla	TCAAgCCTCAgACAgTggTTC
EF1A-F2(panzu)	CACTgAgTgggTggAgACTgAAgTTAg
EF1A-F(zibei)	CTgggAAAgTgATgTCgTgT
EF13	CCAACTTCTCggggACTgTg
EBV-R	gTggTTTgTCCAAACTCATC
DuetUP2	TTgTACACggCCgCATAATC
DuetDOWN1-Primer	gATTATgCggCCgTgTACAA
DsRed1-N	gTACTggAACTggggggACAg
DsRed1-C	AgCTggACATCACCTCCCACAACg
CVN	ggTgggAggTCTATATA
CMV-R	gTTCACggTgCCCTCC
CMV-F	TgggCggTAggCgTgTACggTg
CMV-enhancer-R-chimeng	CTATTggCgTTACTATTgAC
CMV-30	ATAACCCCgCCCCgTTg
CMV-24	TTAggACAAggCTggTgg
CAGR2	gTAACgCCAATAgggACTTTC
BlaRV	ACTCACgTTAAgggATTTTggTCA
BlaM1	AAgCCCTCCCgTATCgTAgTTATC
attB2-LRR	ACCACTTTgTACAAgAAAgCTgggT
attB1-LRF	ACAAgTTTgTACAAAAAAgCAggCT
a-Factor	TACTATTgCCAgCATTgCTgC
ACYCDuetUP1	ggATCTCgACgCTCTCCCT
AC5	ACACAAAgCCgCTCCATCAg
AB-R(aisimai)	CACTgCATTCTAgTTgTgg
AB-R	CAAACTCATCAATgTATCTTATCA
AB-F	TCTACgTCTTTgTTTCgT
AAVS1-Seq-R(group pan)	CAggTAAAACTgACgCACggAgg
AAVS1-Seq-F(group pan)	TTTCCggAgCACTTCCTTCTCg
601SaF-F-chimeng	TTCCTTgACCCTggAAggTg
5BD	TCATCggAAgAgAgTAgT
5AOX1	gACTggTTCCAATTgACAAgC
5AD	AgggATgTTTAATACCACTAC
3BD	TAAgAgTCACTTTAAAATTTgTATAC
3AOX1	ggCAAATggCATTCTgACAT
3AD	AgATggTgCACgATgCACAg
35S	gACgCACAATCCCACTATCC
27F	AgAgTTTgATCCTggCTCAg
-20	TgTgTAgCgCCAAgTgCCCA
1525R	AgAAAggAggTgATCCAgCC
1492R	ggTTACCTTgTTACgACTT
(huijun)BGH-R	gAAAggACAgTgggAgTg