Small RNA sequencing
Service introduction
Small RNAs (sRNA) are important regulators of life activities, playing important roles in gene expression regulation, individual development, metabolism, and the occurrence of diseases. The Illumina sequencing platform can perform deep sequencing of all sRNA in the sample to achieve qualitative and quantitative research purposes. Each sample can obtain more than 10 million sRNA sequencing sequences. Through massive parallel sequencing, it can discover, identify, and quantify the small RNA map at the whole genome level of any species, the excavation of new mRNA molecules, the prediction of target genes, identification, and differential expression analysis between samples.
Technical procedures
Sample requirements
实验类型 | RNA样品总量 | 样品浓度和纯度 | 样品质量 | 样品保存 | 样品运输 |
Small RNA测序 | 一次性提供至少3 μg高质量的总 RNA | 样品浓度 >100 ng/μl,OD260/280 介于 1.8-2.0 之间,无肉眼可见污染 | 使用 Agilent Bioanalyzer 仪器检测,则获得的总的 RNA 28S:18S >1,RIN>7.5 | 请选择乙醇、DEPC 水保存样品,并在样品信息单中注明 | 样品请置于 1.5 ml 管中,并使用封口膜封好,放置足够的干冰运输 |
Experiment cycle and sequencing strategy
实验类型 | 测序策略 | 数据量 | 实验周期 |
Small RNA测序 | HiseqSE50 | 10M | 样品质检合格后,30-50 个工作日 |