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Service introduction




        In recent years, the research on the genomes of pathogenic microorganisms has made rapid progress. Genome research refers to the sequencing of the entire genome of microorganisms, and on the basis of understanding the overall gene structure, the functions of each gene alone or the interaction between several genes are studied. Through genome research, the complete genes of microorganisms are fundamentally revealed, which can not only discover new interactions between genes, new regulatory factors, etc., but also enable humans to master the pathogenic mechanism and laws of pathogenic microorganisms at a higher level. This will lead to the development of new preparations, vaccines and drugs for the diagnosis, prevention and treatment of microbial infections.


        According to different research purposes, microbial genome sequencing is mainly divided into four types: microbial genome survey, microbial genome framework, fine map and finished map. Our company uses the Illumina platform for bacterial genome sequencing, using short-fragment high-coverage sequencing, combined with Illumina paired-end sequencing, to provide personalized solutions for different sequencing studies.


 

 

Assembly evaluation standards



       

Service type

Completion standard

Microbial genome survey

Sequencing depth reaches 50X

Microbial genome framework

Genome coverage is greater than 95%, gene region coverage is greater than 98%

Microbial genome fine map

Genome coverage is greater than 98%, gene region coverage is greater than 99%

 


Technical procedures




       图片5

 

Bioinformatics analysis




生物信息学分析

基因组调查信息分析

基因组精细图信息分析

基因组框架图信息分析

基因组完成图信息分析

原始数据统计       

 原始数据统计

原始数据统计  

原始数据统计         

 序列的初步组装    

  序列组装

 序列组装       

 序列组装    

基因组大小的预测    

  ORF 预测和基本功能注释 

ORF 预测和基本功能注释 

ORF 预测和基本功能注释      

GC 含量和重复序列的预测 

  完成图绘制的建议 

精细图绘制的建议    

比较基因组学和功能基因组学的分析

 

Sample requirements




实验类型

RNA样品总量

样品浓度和纯度

样品保存

样品选择

微生物基因组denovo测序

小片段文库样品制备需要总量 3 μg 的样品,mate-pair 文库(2 kb、5 kb、10 kb)样品制备需要 10-50 μg 的样 

样品浓度 >100 ng/μl,OD260/280 介于 1.8-2.0 之间,无肉眼可见污染 

请选择干粉、酒精、TE 或超纯水的保存方式之一。

对于细菌和真菌样品,希望尽量来自于同一个菌株,不要有宿主 DNA 污染

 


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Guangzhou IGE Biotechnology Co., Ltd.
Email: marketing@igebio.com
QQ: 1787730693
Address: Building G1-903, South China New Materials Innovation Park, #31 Kefeng Road, Huangpu Dist. Guangzhou, Guangdong prov. China 510663

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