Service introduction
In recent years, the research on the genomes of pathogenic microorganisms has made rapid progress. Genome research refers to the sequencing of the entire genome of microorganisms, and on the basis of understanding the overall gene structure, the functions of each gene alone or the interaction between several genes are studied. Through genome research, the complete genes of microorganisms are fundamentally revealed, which can not only discover new interactions between genes, new regulatory factors, etc., but also enable humans to master the pathogenic mechanism and laws of pathogenic microorganisms at a higher level. This will lead to the development of new preparations, vaccines and drugs for the diagnosis, prevention and treatment of microbial infections.
According to different research purposes, microbial genome sequencing is mainly divided into four types: microbial genome survey, microbial genome framework, fine map and finished map. Our company uses the Illumina platform for bacterial genome sequencing, using short-fragment high-coverage sequencing, combined with Illumina paired-end sequencing, to provide personalized solutions for different sequencing studies.
Assembly evaluation standards
Service type | Completion standard |
Microbial genome survey | Sequencing depth reaches 50X |
Microbial genome framework | Genome coverage is greater than 95%, gene region coverage is greater than 98% |
Microbial genome fine map | Genome coverage is greater than 98%, gene region coverage is greater than 99% |
Technical procedures
Bioinformatics analysis
生物信息学分析 | |||
基因组调查信息分析 | 基因组精细图信息分析 | 基因组框架图信息分析 | 基因组完成图信息分析 |
原始数据统计 | 原始数据统计 | 原始数据统计 | 原始数据统计 |
序列的初步组装 | 序列组装 | 序列组装 | 序列组装 |
基因组大小的预测 | ORF 预测和基本功能注释 | ORF 预测和基本功能注释 | ORF 预测和基本功能注释 |
GC 含量和重复序列的预测 | 完成图绘制的建议 | 精细图绘制的建议 | 比较基因组学和功能基因组学的分析 |
Sample requirements
实验类型 | RNA样品总量 | 样品浓度和纯度 | 样品保存 | 样品选择 |
微生物基因组denovo测序 | 小片段文库样品制备需要总量 3 μg 的样品,mate-pair 文库(2 kb、5 kb、10 kb)样品制备需要 10-50 μg 的样 | 样品浓度 >100 ng/μl,OD260/280 介于 1.8-2.0 之间,无肉眼可见污染 | 请选择干粉、酒精、TE 或超纯水的保存方式之一。 | 对于细菌和真菌样品,希望尽量来自于同一个菌株,不要有宿主 DNA 污染 |