The kit utilizes magnetic bead purification technology, suitable for extracting high-purity total DNA from plant samples/seeds/processed samples. The obtained DNA can be directly used for PCR, quantitative fluorescent PCR, restriction enzyme digestion, Southern blotting, and other experiments. This product has been successfully used in transgenic detection projects at multiple entry-exit inspection and quarantine bureaus.

        The kit has been successfully operated on the KingFisher FLEX\ ML\DUO nucleic acid extraction instrument, and it can also be used with instruments such as Xi'an Tianlong Technology Co., Ltd. NP968, Shanghai Zhijiang MP-120, Circular Nano Smart LabAssist-16, and Daan Gene Smart2000. With the KingFisher Flex automated nucleic acid extraction instrument, the purification of 96 samples can be completed in just 30 minutes.

        As shown in the figures:

1-4：水稻叶，花生叶，番茄叶，黄豆叶

（1)-(4)水稻叶，花生叶，番茄叶，黄豆叶

        Rice leaves, peanut leaves, tomato leaves, soybean leaves (1)-(4).

        Four fresh leaf samples (50 mg) were respectively extracted using centrifugal column method from a certain Q company ((1)-(4)) and iPure Plant DNA Kit ((1)-(4)). Then, 5% purified DNA samples were loaded onto a 1% agarose gel for electrophoresis analysis (left figure). The extraction instrument used was the KingFisher FLEX.

         After the DNA extraction, 1% of the nucleic acid was taken as the template for amplifying the ITS sequence, followed by 1% agarose gel electrophoresis. From the brightness of the electrophoresis, it can be seen that the extraction effect of the iPure Plant DNA Kit with the matched nucleic acid extraction instrument completely replaces the centrifugal column method, with a shorter total time, less labor, and simpler operation.

        Four fresh seed leaf samples (50 mg) were extracted using the iPure Plant DNA Kit and Q company Cat.NO.67163, and the purified DNA was measured using ThermoFisher Nanodrop 2000. The results from the nucleic acid extraction instrument tested by fluorescent quantitative PCR are shown in the figure: highly consistent and repeatable.