The iPure Bacterial DNA & RNA Kits utilize silica column purification to efficiently extract high-purity DNA or RNA from bacterial cultures or other samples. Operators can complete the extraction of multiple samples within 60 minutes.

        Experiment Example: Genomic DNA Extraction from Gram-Positive Bacteria

        Using this kit, high-purity genomic DNA was extracted from Candida albicans and Staphylococcus aureus, as shown in the agarose gel electrophoresis results.

        Experiment Example: Genomic DNA Extraction from Gram-Negative Bacteria

        Similarly, high-purity genomic DNA was extracted from Escherichia coli and Klebsiella pneumoniae using the same kit, as evidenced by the agarose gel electrophoresis results.

     iPure Bacteria DNA & RNA M.tuberculosis Kits (Centrifugal Column Method)       

        Specifically designed for difficult-to-lyse bacteria such as Staphylococcus aureus, Enterococcus faecalis, and Mycobacterium tuberculosis, this kit utilizes zirconium dioxide magnetic beads to break down the tough cell wall efficiently. After brief centrifugation and treatment with lysozyme, high-quality DNA can be obtained, suitable for downstream molecular biology analyses, particularly in M.tuberculosis DNA diagnostics research.

        Cell Wall Structure of M.tuberculosis

        Mycobacterium species have a high lipid content in their cell walls, mainly comprising mycolic acids, which can hinder dye penetration. Acid-fast staining methods, such as Ziehl-Neelsen staining, are typically used to visualize these bacteria.

      iPure Bacterial DNA & RNA Magic Beads Kits (Magnetic Bead Method)  

        This kit is suitable for high-throughput sample extraction, utilizing lysozyme to break down the bacterial cell wall and high-concentration guanidine salts to lyse the cells. Silica hydroxylated magnetic beads are then used to bind bacterial DNA efficiently, facilitating nucleic acid separation. The average processing time per sample is less than 5 minutes.

        Please refer to the provided images for the experimental results and further details on the products.