The iPure Tissue DNA kit utilizes magnetic bead purification technology to extract high-purity total DNA from tissue or cell samples. The extracted DNA can be directly used for PCR, quantitative PCR, Southern Blot, and other experiments. This product has been successfully operated on KingFisher FLEX, ML, DUO nucleic acid extractors, as well as compatible instruments from Xi'an Tianlong Technology Co., Ltd., Shanghai Zhijiang MP-120, Nanodot Smart LabAssist-16, Daan Gene Smart2000, and others. The KingFisher Flex automated nucleic acid extractor can complete the purification of 96 samples in just 30 minutes.
Key Features:
Increased Yield: 5-10 times higher compared to standard methods
Lysis Time: <3 hours
High Integrity DNA: Suitable for PCR and NGS
Complete Removal of Inhibitors
Automation Compatibility: Can be automated on KingFisher
Results:
Gel electrophoresis results show comparable extraction efficiency between iPure Tissue DNA Kit and the imported product QIAGEN, for both fresh and frozen samples of liver, kidney, and spleen tissues from mice.
Additional Features:
High-quality DNA Amplification: PCR amplification of single-copy genes (850 bp fragment) from bovine tissues and GAPDH gene (266 bp fragment) from mouse tail tissues purified using iPure Tissue DNA Kit demonstrated successful PCR amplification.
Gel electrophoresis results (images not provided) showed the presence of the desired PCR products.