The iPure Plasmid DNA Extraction Kit is designed for the large-scale purification of various endotoxin-free plasmids (plasmid, cosmid) from bacterial cultures. The kit utilizes the traditional SDS alkaline lysis method combined with a specific combination of silica membrane and special buffers to effectively remove endotoxins, offering high efficiency, speed, and convenience. With this kit, high-purity endotoxin-free plasmid DNA (OD260/OD280=1.82.0, endotoxin content <0.1 EU/μg) can be purified from 100 ml LB overnight cultures, yielding 500 μg1 mg of DNA. This purified DNA is suitable for molecular biology experiments such as cell transfection and restriction enzyme digestion.

 标准曲线的制备

        Utilizes the Xiamen Hai Bioscience Co., Ltd. colorimetric assay kit to detect residual endotoxins in plasmids.

        A standard curve is prepared using Thermofisher MK3 to quantify endotoxin levels in plasmid DNA.

Gel electrophoresis results demonstrate differences in plasmid transfection efficiency and transfection time using different kits.

磁珠分选试剂盒

        A kit designed for NGS (Next Generation Sequencing) nucleic acid size range selection, such as selecting fragments ranging from 100 to 750 bp from fragmented gDNA.

        Enables size selection without gel cutting, offering convenience and compatibility with both manual and automated workstations.

Key Features

        Convenience: Simplifies size selection without gel cutting.

        Wide Range Selection: Offers a broader range of nucleic acid size selection compared to similar products.

        Compatibility: Suitable for use with both manual and automated workstations.

        High Throughput: Enables simultaneous processing of 96 samples with automated workstations.

Different ratios of magnetic bead solutions are evaluated for magnetic bead recovery, demonstrating the capture of fragments above 100 bp when the concentration is 1.0×.

Ultrasonic smear DNA fragments are subjected to selection, and the fragment sizes are analyzed using Agilent 2100 high-sensitivity chips,

showing accurate and successful size selection with narrow peak widths.

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