Real-time quantitative PCR (qPCR) services

Platform introduction

       Real-time quantitative polymerase chain reaction (qPCR) is a method that uses fluorescence to monitor the progress of PCR in real time. This allows for accurate and precise quantification of target DNA or RNA.

Service advantages

        Short turnaround time: Results are typically available within 3-5 business days.

        Comprehensive customer service

        Clear and reasonable pricing

Related methods

        There are two main types of fluorescent probes used in qPCR: SYBR Green and TaqMan.

        SYBR Green: SYBR Green is a dye that binds to double-stranded DNA and emits fluorescence. As the PCR reaction progresses, the amount of fluorescence increases in proportion to the amount of DNA amplified.

        TaqMan: TaqMan probes are designed to hybridize to a specific DNA sequence and contain a fluorescent reporter and a quencher. As the PCR reaction progresses, the probe is cleaved by the Taq polymerase, releasing the fluorescent reporter and resulting in an increase in fluorescence.

Services offered

        IGEBio offers a comprehensive range of qPCR services, including:

        Designing quantitative primers

        Extracting total RNA and reverse transcribing it to cDNA

        Performing real-time PCR

        Analyzing experimental results

Technical process

Sample requirements

        Fresh, high-quality materials, or purified total RNA

        Known gene sequence, GenBank accession number, detailed reference, sample source, etc.

Part of the technical map display

        Relative quantification

        Absolute quantification (including standard curve)

        SYBR Green staining (including melting curve)

        TaqMan probes (probe synthesis)

Standard curve

Melting curve

Amplification curve

Genotype map

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QPCR service information table