Gene Editing

Service introduction

CRISPR (Clustered regularly interspaced short palindromic repeats) and Cas (CRISPR associated genes) is an efficient bacterial immune defense system that bacteria use to protect themselves against viruses. CRISPR/Cas systems are of three types: I, II, and III. The II type is relatively simple, consisting of the Cas9 protein and guide RNA (gRNA), and is also the type that has been studied most deeply.

CRISPR/Cas9 systems encode small RNA and RNA-guided DNA nucleases to identify and cut DNA to degrade foreign nucleic acids. Researchers have modified it as a precise universal gene weapon that can be used to delete, add, activate, or inhibit the target genes of other organisms. CRISPR/Cas9 systems are currently the most widely used and efficient gene editing tools.

Service Advantages

High accuracy: Considering the characteristics of CRISPR/Cas9 systems and their binding and cutting of target DNA, gRNAs with high specificity and the lowest off-target rate are designed

Short experimental cycle: Years of project experience make the construction and use of CRISPR/Cas9 systems extremely convenient, reducing experimental difficulty and shortening the experimental cycle.

Multiple editing capabilities: Multiple target sites can be quickly and simultaneously edited using viruses, Cas9 mRNA, or protein/gRNA systems.

Rich functions: It can achieve a variety of purposes such as knockout, insertion, inhibition, and activation.

Services provided

CRISPR-Cas9 sgRNA design: Provides sgRNA design and synthesis for a variety of model animals, and selects the sgRNA with the lowest off-target efficiency.
sgRNA synthesis service: AegiBio can provide you with a large number of in vitro transcribed gRNA.
CRISPR-Cas9 sgRNA library construction: Design, chip synthesis, and cloning construction services for sgRNA libraries for a variety of model animals.
**CRISPR-Cas9 sgRNA libraries can be widely used for rapid and efficient screening of multiple drug targets or pathogenic genes.
CRISPR-Cas9 lentivirus packaging service:
Stable cell line construction and screening service:
Mouse gene knockout service: