sgRNA synthesis

Service introduction

       sgRNA is a high-purity RNA synthesized in vitro that can bind to Cas9 protein to form a complex. The complex can be introduced into cells by electroporation, liposome transfection, or microinjection, and then enters the cell nucleus through the nuclear localization signal peptide (NLS) to perform gene editing.

        Compared with other methods of introducing editing elements, the gene editing method of introducing Cas9/sgRNA complex has several important advantages:

        All elements are in mature form, and do not need to be transcribed and translated. Therefore, there are no problems such as codon incompatibility, transcription regulation, and low transcription efficiency. It is suitable for various cells of various organisms, including stem cells and fertilized eggs, to maximize the success of editing.

        There is no risk of integration of editing elements into the genome. Cas9 and sgRNA are both metabolized by cells and will eventually be metabolized and disappear without leaving a trace. This is especially important in situations where animal trials or clinical trials are required. Similarly, it is also critical if multiple rounds of editing/screening are required.

        The introduction of the Cas9/sgRNA complex has higher editing specificity.

        Convenient and efficient. After the target sequence is determined, the preparation of the Cas9/sgRNA complex can be completed within 3 working days. It can be paralleled at high throughput, and can also be prepared in large quantities at the clinical scale.

        Please specify the 20 bases of the target DNA sequence. AegiBio provides the corresponding sgRNA. sgRNA synthesis uses in vitro transcription, which has the advantages of good biocompatibility, high purity, and high activity. It can also be expanded to g-level synthesis. A single reaction can yield >100ug sgRNA. The synthesis cycle is 3 working days.

        IGEBio's customized sgRNA synthesis is designed for high-requirement basic research and clinical translation. It has ultra-low endotoxin content (meets the human injection standard) and is the best reagent for research and clinical application development. sgRNA is a RNase-free aqueous solution. Because RNA is easy to degrade, it must be stored at -80℃. When using it, it is necessary to strictly follow the requirements for operating RNA. This reagent is a sterile, RNase-free, and DNase-free reagent that can be used directly for cell culture or animal experiments.

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